1. To access information on O-GlcNAcylated proteins in different brain structures of Drosophila (e.g. Mushroom Body), follow the flowchart provided.

2. To determine whether proteins of interest are O-GlcNAcylated in various brain structures, follow the flowchart provided.

3.  In the search results, the term "Score" refers to the normalized O-GlcNAc level.The following flowchart shows the strategy to normalize the O-GlcNAcylated protein level with expression level for each candidate substrate in different brain regions. A normalizing factor for each substrate in a given brain region was calculated using single cell RNA-seq expression data, and the normalized O-GlcNAc level was determinted as the log2 FC divided by its normalizing factor. 

 4. Furthermore, the database also provides links to the information about each protein on the UniProt and FlyBase websites.