The O-GlcNAcylated proteome of tsOGA was mapped by TurboID-CpOGACD which is a new O-GlcNAcylation profiling tool. By combining the catalytic-dead mutant CpOGAD298N (CpOGA CD) that recognizes O-GlcNAcylated proteins without removing the GlcNAc moiety with proximity labeling enzyme TurboID, we developed an O-GlcNAcylation profiling tool that translates O-GlcNAc modification into biotin conjugation for tissue-specific substrates enrichment and Mass Spectrometry (MS) identification. Once induced by different tissue-specific drivers, this tool could tag and enrich O-GlcNAc substrates in a tissue-specific manner. Meanwhile, CpOGAD298N D401A (CpOGADM) was adopted as control to distinguish the O-GlcNAc independent protein-protein interactions.
Citation
For publication of the results in this database, please cite the following articles:
Yu H, Liu D, Zhang Y, Tang R, Fan X, Mao S, Lv L, Chen F, Qin H, Zhang Z, van Alten DMF, Yang B, Yuan K. Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning. Elife. 2024 Apr 15;13:e91269. doi: 10.7554/eLife.91269. PMID: 38619103.
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If you have any questions about the tsOGA, please contact us:
Kai Yuan (Corresponding author): yuankai@csu.edu.cn Haibin Yu (Author): yuhaibin@csu.edu.cn
Department:
Hunan Key Laboratory of Molecular Precision Medicine, Department of Oncology, Xiangya Hospital & Center for Medical Genetics, School of Life Sciences, Changsha, Hunan, China
